General Cell Lines and Cell Cultures
ATCC Pseudomonas aeruginosa
Pseudomonas aeruginosa strain PRD-10 [CIP 103467, NCIB 10421, PCI 812] is a whole genome-sequenced strain that was isolated from a water bottle in an animal room. This bacterium can be used in disinfectant testing, antimicrobial preservative assays, testing of antimicrobial handwashing formulations, testing of slimicide, and quality control of Biosynth products. It is recommended by ATCC for use in the test described in ASTM Standard Test Method E686-91 where only the taxon is specified.
ATCC pRS316 phagemid; in E. coli
This is one of a series of pBluescript-based YC-type (centromeric) yeast shuttle vectors (ATCC 77142-77145) differing in the yeast selectable marker gene. It encodes the beta galactosidase alpha peptide for blue-white color detection of inserts and also has the T7 and T3 promoters for in vitro RNA synthesis and priming sites for sequencing. It is useful in plasmid shuffling experiments. It was constructed by inserting a 1.112 kb fragment containing the URA3 gene into the NdeI site of the pRSS56 vector and also inserting a cassette containing CEN6 and the ARS associated with histone 4 (ARSH4) into the AatII site of the same vector. All ends were blunted. The pRSS56 vector was constructed by ligating a PvuI fragment (bp 498-2412) of pBluescript KS+ and the fragment from the unique NdeI and AatII sites between bla and f1 origin of pBS(+). The order of the major features in this plasmid is: URA3 ? f1 ori (NaeI) ? T7 promoter ? lacZ?/MCS ? T3 promoter ? pMB1 ori ? bla ? CEN6 ? ARSH4.
Maine Molecular Quality Controls Inc QIAstat-Dx GI 2 Control Panel
QIAstat-Dx GI 2 Control Panel is intended for use as reference material to monitor the performance of in vitro laboratory nucleic acid testing procedures for the qualitative detection of bacterial, viral, and parasitic pathogens the QIAstat-Dx Gastrointestinal Panel 2 performed on QIAstat-Dx systems. QIAstat-Dx GI 2 Control Panel is composed of 3 controls, QIAstat-Dx GI 2 Positive A, QIAstat-Dx GI 2 Positive B, and QIAstat-Dx GI 2 Negative. QIAstat-Dx GI 2 Positive A and QIAstat-Dx GI 2 Positive B contain surrogate control material composed of synthetic nucleic acid corresponding to genome segments of pathogens. QIAstat-Dx GI 2 Negative contains no nucleic acid.
ADDEXBIO TECHNOLOGIES HACAT CELLS (T0020001
In vitro spontaneously transformed keratinocytes from histologically normal skin. Species: Homo sapiens. Keratinocyte. Adherent monolayer. Patient: Male, Caucasian, 62 years of age. Medium: AddexBio-Optimized DMEM (C0003-02) + 10% FBS. Subculture: Recommended 1:5 to 1:10 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 degrees Celsius. Bacteria: Negative. Yeast: Negative. Mycoplasma: Negative. HIV: Negative. Hepatitis B: Negative. Hepatitis C: Negative.
Zymo Research Corporation Mix & Go! Competent Cells - DH5 Alpha, 96 x 50 µl
Mix & Go E. coli cells are made chemically competent by a unique method that completely eliminates the need for heat shock and related procedures. For transformation, DNA can be added directly to Mix & Go cells and the mixture spread directly to a culture plate. Transformation efficiencies typically range from 108-109 transformants/µg of pUC19 DNA , which make the cells optimal for cloning, sub-cloning, library construction, etc. Premade Mix & Go cells are supplied as a pack of 10 convenient 100 µl/tube aliquots or in a 96-well format (12 x 8 - tube strips) of 50 µl/tube.
Lonza Walkersville EGM Endothelial Cell Growth Medium BulletKit
Choose from a variety of endothelial cell growth media for an easy cultivation of primary endothelial cells. Every endothelial cell growth medium has its special formulation and is optimized to grow certain cell types. Each type of endothelial cell growth medium is guaranteed to perform when used to grow Clonetics Primary Cells. Content:1 x EBM Basal Medium (CC-3121), 500 mL1 x EGM SingleQuots Supplement Pack (CC-4133) containing:1 x Green Cap Vial with hEGF 0.50 mL1 x Natural Cap Vial with Hydrocortisone 0.50 mL1 x Red Cap Vial with GA-1000 0.50 mL1 x Bottle BBE 2.00 mL1 x Blue Cap Vial with Ascorbic Acid 0.50 mL1 x Bottle FBS 10.00 mL
ADDEXBIO TECHNOLOGIES MOLM-13 Cells, Human AML Cell Line, 1 x 10^5 viable cells/mL, 20% FBS in RPMI-1640 Medium, Suspension Growth
MOLM-13 Cells, Human AML Cell Line, 1 x 10^5 viable cells/mL, 20% FBS in RPMI-1640 Medium, Suspension Growth
iQ Biosciences New Zealand White Rabbit PBMCs, 5M cells/vial
High-quality, high-viability (> 80%) peripheral blood mononuclear cells (PBMCs) from the New Zealand white (NZW) rabbit, a commonly-used outbred rabbit strain. Used for a wide variety of immunology-based applications, including in-vitro immunoassays and ex-vivo applications. All orders come with an iQ Certificate of Analysis.
Lonza Walkersville NHDF-Ad - Human Dermal Fibroblasts, Adult
Human Dermal Fibroblasts are available from either adult skin (NHDF-Ad) or neonatal foreskin (NHDF-Neo). They are derived from the dermis of normal human neonatal foreskin or adult skin. They are cryopreserved at the end of primary culture. Normal Human Epidermal Keratinocytes (00192627) from matched donors are available for certain lots.Lonza's human dermal fibroblasts are guaranteed through 15 population doublings when using FGM-2 Growth Media. Dermal fibroblasts are characterized by morphological observation throughout serial passage.All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.Applications:ECM protein analysisWound healingCollagen metabolismCosmeticsSkin therapy/models Content:1 x Cryopreserved ampule of NHDF-Ad containing ≥ 500,000 cells
Sciencell Research Laboratories Human Astrocytes 1 x 10^6 cells in 1 ml volume.
Isolated from human brain (cerebral cortex). HA are cryopreserved at passage one and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. Storage: Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments.